ALS Disease Modelling with Patient-Derived iPSC - an Interview with Dr Rosa, University of Rome

Cell health in routine culture is key to successful downstream applications: “at the beginning of the differentiation the cells must be healthy, if you have a lot of cell death in your cultures, the differentiation will start on the wrong foot.”
In this interview Dr. Alessandro Rosa offers insight and tips for working with high cell quality standards for expansion and differentiation of iPSC to fates such as motor neurons and skeletal muscle.
He shares crucial points to consider when adapting differentiation protocols, and differences to expect when switching maintenance media, including how culture conditions impact downstream result.